Construct: shRNA TRCN0000328981

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026240.2-1364s21c1
DNA Barcode:
CCAAGAACTTACAGCTAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gramd3 (107022)
Gene Description:
GRAM domain containing 3
Transcript:
RefSeq NM_026240.2 (CURRENT)
Match location:
Position 1364 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000328981)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGAACTTACAGCTAACAT
Hairpin Sequence:
5'-CCGG-CCAAGAACTTACAGCTAACAT-CTCGAG-ATGTTAGCTGTAAGTTCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGAACTTACAGCTAACATCTCGAGATGTTAGCTGTAAGTTCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGAACTTACAGCTAACATCTCGAGATGTTAGCTGTAAGTTCTTGG-3'

Other clones with same target sequence:

  1. TRCN0000192756