Construct: shRNA TRCN0000328997

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011365.2-5375s21c1
DNA Barcode:
CTAAATTGCTAGCAATCTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Itsn2 (20403)
Gene Description:
intersectin 2
Transcript:
RefSeq NM_011365.2 (NON-CURRENT)
Match location:
Position 5375 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000328997)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAAATTGCTAGCAATCTATG
Hairpin Sequence:
5'-CCGG-CTAAATTGCTAGCAATCTATG-CTCGAG-CATAGATTGCTAGCAATTTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAAATTGCTAGCAATCTATGCTCGAGCATAGATTGCTAGCAATTTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAAATTGCTAGCAATCTATGCTCGAGCATAGATTGCTAGCAATTTAG-3'

Other clones with same target sequence:

(none)