Construct: shRNA TRCN0000328998

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011365.2-293s21c1
DNA Barcode:
GCCAAATATGTGGGCTATTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Itsn2 (20403)
Gene Description:
intersectin 2
Transcript:
RefSeq NM_011365.2 (NON-CURRENT)
Match location:
Position 293 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000328998)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAATATGTGGGCTATTAC
Hairpin Sequence:
5'-CCGG-GCCAAATATGTGGGCTATTAC-CTCGAG-GTAATAGCCCACATATTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAATATGTGGGCTATTACCTCGAGGTAATAGCCCACATATTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAATATGTGGGCTATTACCTCGAGGTAATAGCCCACATATTTGGC-3'

Other clones with same target sequence:

(none)