Construct: shRNA TRCN0000329017

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009384.3-1262s21c1
DNA Barcode:
CTCAAGCTACTGCCGGAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tiam1 (21844)
Gene Description:
T cell lymphoma invasion and metastasis 1
Transcript:
RefSeq NM_009384.3 (CURRENT)
Match location:
Position 1262 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000329017)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAAGCTACTGCCGGAATTT
Hairpin Sequence:
5'-CCGG-CTCAAGCTACTGCCGGAATTT-CTCGAG-AAATTCCGGCAGTAGCTTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAAGCTACTGCCGGAATTTCTCGAGAAATTCCGGCAGTAGCTTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAAGCTACTGCCGGAATTTCTCGAGAAATTCCGGCAGTAGCTTGAG-3'

Other clones with same target sequence:

(none)