Construct: shRNA TRCN0000329070

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172510.4-1434s21c1
DNA Barcode:
GAGGACATCCTCCAGTATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mfsd4a (213006)
Gene Description:
major facilitator superfamily domain containing 4A
Transcript:
RefSeq NM_172510.4 (CURRENT)
Match location:
Position 1434 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000329070)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGACATCCTCCAGTATAAA
Hairpin Sequence:
5'-CCGG-GAGGACATCCTCCAGTATAAA-CTCGAG-TTTATACTGGAGGATGTCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGACATCCTCCAGTATAAACTCGAGTTTATACTGGAGGATGTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGACATCCTCCAGTATAAACTCGAGTTTATACTGGAGGATGTCCTC-3'

Other clones with same target sequence:

(none)