Construct: shRNA TRCN0000329145

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018860.3-214s21c1
DNA Barcode:
GAGTCTCAGTAGACCTGGAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rpl41 (67945)
Gene Description:
ribosomal protein L41
Transcript:
RefSeq NM_018860.3 (NON-CURRENT)
Match location:
Position 214 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000329145)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTCTCAGTAGACCTGGAAC
Hairpin Sequence:
5'-CCGG-GAGTCTCAGTAGACCTGGAAC-CTCGAG-GTTCCAGGTCTACTGAGACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTCTCAGTAGACCTGGAACCTCGAGGTTCCAGGTCTACTGAGACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTCTCAGTAGACCTGGAACCTCGAGGTTCCAGGTCTACTGAGACTC-3'

Other clones with same target sequence:

(none)