Construct: shRNA TRCN0000329291

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007652.4-349s21c1
DNA Barcode:
TTGTCATGGTGAGATCATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cd59a (12509)
Gene Description:
CD59a antigen
Transcript:
RefSeq NM_007652.4 (NON-CURRENT)
Match location:
Position 349 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000329291)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGTCATGGTGAGATCATTAT
Hairpin Sequence:
5'-CCGG-TTGTCATGGTGAGATCATTAT-CTCGAG-ATAATGATCTCACCATGACAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGTCATGGTGAGATCATTATCTCGAGATAATGATCTCACCATGACAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGTCATGGTGAGATCATTATCTCGAGATAATGATCTCACCATGACAA-3'

Other clones with same target sequence:

(none)