Construct: shRNA TRCN0000329400

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028873.4-1456s21c1
DNA Barcode:
TGAACTAAAGAAGGCCTATAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dnajc14 (74330)
Gene Description:
DnaJ heat shock protein family (Hsp40) member C14
Transcript:
RefSeq NM_028873.4 (CURRENT)
Match location:
Position 1456 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000329400)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAACTAAAGAAGGCCTATAG
Hairpin Sequence:
5'-CCGG-TGAACTAAAGAAGGCCTATAG-CTCGAG-CTATAGGCCTTCTTTAGTTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAACTAAAGAAGGCCTATAGCTCGAGCTATAGGCCTTCTTTAGTTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAACTAAAGAAGGCCTATAGCTCGAGCTATAGGCCTTCTTTAGTTCA-3'

Other clones with same target sequence:

(none)