Construct: shRNA TRCN0000329414

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018887.3-1033s21c1
DNA Barcode:
ATTCTGGACACTTGGATATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp39a1 (56050)
Gene Description:
cytochrome P450, family 39, subfamily a, polypeptide 1
Transcript:
RefSeq NM_018887.3 (NON-CURRENT)
Match location:
Position 1033 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000329414)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTCTGGACACTTGGATATAT
Hairpin Sequence:
5'-CCGG-ATTCTGGACACTTGGATATAT-CTCGAG-ATATATCCAAGTGTCCAGAAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTCTGGACACTTGGATATATCTCGAGATATATCCAAGTGTCCAGAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTCTGGACACTTGGATATATCTCGAGATATATCCAAGTGTCCAGAAT-3'

Other clones with same target sequence:

(none)