Construct: shRNA TRCN0000329689

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001674.2-659s21c1
DNA Barcode:
GCTGAACTGAAGGCTCAGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATF3 (467)
Gene Description:
activating transcription factor 3
Transcript:
RefSeq NM_001674.2 (NON-CURRENT)
Match location:
Position 659 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000329689)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAACTGAAGGCTCAGATT
Hairpin Sequence:
5'-CCGG-GCTGAACTGAAGGCTCAGATT-CTCGAG-AATCTGAGCCTTCAGTTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAACTGAAGGCTCAGATTCTCGAGAATCTGAGCCTTCAGTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAACTGAAGGCTCAGATTCTCGAGAATCTGAGCCTTCAGTTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000013572