Construct: shRNA TRCN0000329764

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005230.2-1476s21c1
DNA Barcode:
GCTTTCTTCAAACTCTCAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ELK3 (2004)
Gene Description:
ETS transcription factor ELK3
Transcript:
RefSeq NM_005230.2 (NON-CURRENT)
Match location:
Position 1476 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000329764)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTCTTCAAACTCTCAGAA
Hairpin Sequence:
5'-CCGG-GCTTTCTTCAAACTCTCAGAA-CTCGAG-TTCTGAGAGTTTGAAGAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTCTTCAAACTCTCAGAACTCGAGTTCTGAGAGTTTGAAGAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTCTTCAAACTCTCAGAACTCGAGTTCTGAGAGTTTGAAGAAAGC-3'

Other clones with same target sequence:

  1. TRCN0000013879