Construct: shRNA TRCN0000329787

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004516.3-1335s21c1
DNA Barcode:
CCTTCCAAGATGCCCAAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ILF3 (3609)
Gene Description:
interleukin enhancer binding factor 3
Transcript:
RefSeq NM_004516.3 (NON-CURRENT)
Match location:
Position 1335 (CDS)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO_TRC005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000329787)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCCAAGATGCCCAAGAAA
Hairpin Sequence:
5'-CCGG-CCTTCCAAGATGCCCAAGAAA-CTCGAG-TTTCTTGGGCATCTTGGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCCAAGATGCCCAAGAAACTCGAGTTTCTTGGGCATCTTGGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCCAAGATGCCCAAGAAACTCGAGTTTCTTGGGCATCTTGGAAGG-3'

Other clones with same target sequence:

  1. TRCN0000014579