Construct: shRNA TRCN0000329885

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003075.3-513s21c1
DNA Barcode:
GCCTGTCTCGACCTAACATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMARCC2 (6601)
Gene Description:
SWI/SNF related, matrix associated, actin dependent regulator of chromatin subfamily c member 2
Transcript:
RefSeq NM_003075.3 (NON-CURRENT)
Match location:
Position 513 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000329885)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGTCTCGACCTAACATTT
Hairpin Sequence:
5'-CCGG-GCCTGTCTCGACCTAACATTT-CTCGAG-AAATGTTAGGTCGAGACAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGTCTCGACCTAACATTTCTCGAGAAATGTTAGGTCGAGACAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGTCTCGACCTAACATTTCTCGAGAAATGTTAGGTCGAGACAGGC-3'

Other clones with same target sequence:

(none)