Construct: shRNA TRCN0000329894

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005649.2-2122s21c1
DNA Barcode:
TTAGCCCTCATTAGGTATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF354A (6940)
Gene Description:
zinc finger protein 354A
Transcript:
RefSeq NM_005649.2 (NON-CURRENT)
Match location:
Position 2122 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000329894)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTAGCCCTCATTAGGTATTTA
Hairpin Sequence:
5'-CCGG-TTAGCCCTCATTAGGTATTTA-CTCGAG-TAAATACCTAATGAGGGCTAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTAGCCCTCATTAGGTATTTACTCGAGTAAATACCTAATGAGGGCTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTAGCCCTCATTAGGTATTTACTCGAGTAAATACCTAATGAGGGCTAA-3'

Other clones with same target sequence:

(none)