Construct: shRNA TRCN0000329968

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015125.3-4114s21c1
DNA Barcode:
CTTTGCTGAGTTGCCTGAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CIC (23152)
Gene Description:
capicua transcriptional repressor
Transcript:
RefSeq NM_015125.3 (NON-CURRENT)
Match location:
Position 4114 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000329968)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTGCTGAGTTGCCTGAGTT
Hairpin Sequence:
5'-CCGG-CTTTGCTGAGTTGCCTGAGTT-CTCGAG-AACTCAGGCAACTCAGCAAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTGCTGAGTTGCCTGAGTTCTCGAGAACTCAGGCAACTCAGCAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTGCTGAGTTGCCTGAGTTCTCGAGAACTCAGGCAACTCAGCAAAG-3'

Other clones with same target sequence:

  1. TRCN0000018921