Construct: shRNA TRCN0000329978

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013400.3-2170s21c1
DNA Barcode:
CACCAGAAGAAGCACGATGTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
REPIN1 (29803)
Gene Description:
replication initiator 1
Transcript:
RefSeq NM_013400.3 (CURRENT)
Match location:
Position 2170 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000329978)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCAGAAGAAGCACGATGTC
Hairpin Sequence:
5'-CCGG-CACCAGAAGAAGCACGATGTC-CTCGAG-GACATCGTGCTTCTTCTGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCAGAAGAAGCACGATGTCCTCGAGGACATCGTGCTTCTTCTGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCAGAAGAAGCACGATGTCCTCGAGGACATCGTGCTTCTTCTGGTG-3'

Other clones with same target sequence:

(none)