Construct: shRNA TRCN0000330008

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021145.3-983s21c1
DNA Barcode:
TAAGGATTCTCTGACTAATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DMTF1 (9988)
Gene Description:
cyclin D binding myb like transcription factor 1
Transcript:
RefSeq NM_021145.3 (CURRENT)
Match location:
Position 983 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000330008)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAAGGATTCTCTGACTAATAA
Hairpin Sequence:
5'-CCGG-TAAGGATTCTCTGACTAATAA-CTCGAG-TTATTAGTCAGAGAATCCTTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAAGGATTCTCTGACTAATAACTCGAGTTATTAGTCAGAGAATCCTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAAGGATTCTCTGACTAATAACTCGAGTTATTAGTCAGAGAATCCTTA-3'

Other clones with same target sequence:

(none)