Construct: shRNA TRCN0000330026

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024772.3-3055s21c1
DNA Barcode:
CCAAGGATTAGATACTATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZMYM1 (79830)
Gene Description:
zinc finger MYM-type containing 1
Transcript:
RefSeq NM_024772.3 (NON-CURRENT)
Match location:
Position 3055 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000330026)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGGATTAGATACTATATT
Hairpin Sequence:
5'-CCGG-CCAAGGATTAGATACTATATT-CTCGAG-AATATAGTATCTAATCCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGGATTAGATACTATATTCTCGAGAATATAGTATCTAATCCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGGATTAGATACTATATTCTCGAGAATATAGTATCTAATCCTTGG-3'

Other clones with same target sequence:

(none)