Construct: shRNA TRCN0000330045

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002876.2-323s21c1
DNA Barcode:
AGCATACCCAGGGCTTCATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAD51C (5889)
Gene Description:
RAD51 paralog C
Transcript:
RefSeq NM_002876.2 (NON-CURRENT)
Match location:
Position 323 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000330045)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCATACCCAGGGCTTCATAA
Hairpin Sequence:
5'-CCGG-AGCATACCCAGGGCTTCATAA-CTCGAG-TTATGAAGCCCTGGGTATGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCATACCCAGGGCTTCATAACTCGAGTTATGAAGCCCTGGGTATGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCATACCCAGGGCTTCATAACTCGAGTTATGAAGCCCTGGGTATGCT-3'

Other clones with same target sequence:

(none)