Construct: shRNA TRCN0000330087

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021639.4-2021s21c1
DNA Barcode:
CCATGCAAATGGGAACAAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPBP1L1 (60313)
Gene Description:
GC-rich promoter binding protein 1 like 1
Transcript:
RefSeq NM_021639.4 (NON-CURRENT)
Match location:
Position 2021 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000330087)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGCAAATGGGAACAAATT
Hairpin Sequence:
5'-CCGG-CCATGCAAATGGGAACAAATT-CTCGAG-AATTTGTTCCCATTTGCATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGCAAATGGGAACAAATTCTCGAGAATTTGTTCCCATTTGCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGCAAATGGGAACAAATTCTCGAGAATTTGTTCCCATTTGCATGG-3'

Other clones with same target sequence:

  1. TRCN0000146336