Construct: shRNA TRCN0000330087
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_021639.4-2021s21c1
- DNA Barcode:
- CCATGCAAATGGGAACAAATT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GPBP1L1 (60313)
- Gene Description:
- GC-rich promoter binding protein 1 like 1
- Transcript:
- RefSeq NM_021639.4 (NON-CURRENT)
- Match location:
- Position 2021 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000330087)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCATGCAAATGGGAACAAATT
- Hairpin Sequence:
-
5'-CCGG-CCATGCAAATGGGAACAAATT-CTCGAG-AATTTGTTCCCATTTGCATGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCATGCAAATGGGAACAAATTCTCGAGAATTTGTTCCCATTTGCATGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCATGCAAATGGGAACAAATTCTCGAGAATTTGTTCCCATTTGCATGG-3'
Other clones with same target sequence:
-
TRCN0000146336
