Construct: shRNA TRCN0000330231

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006832.2-2351s21c1
DNA Barcode:
GCCTTACCAGACCAGTTAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FERMT2 (10979)
Gene Description:
fermitin family member 2
Transcript:
RefSeq NM_006832.2 (NON-CURRENT)
Match location:
Position 2351 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000330231)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTACCAGACCAGTTAATA
Hairpin Sequence:
5'-CCGG-GCCTTACCAGACCAGTTAATA-CTCGAG-TATTAACTGGTCTGGTAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTACCAGACCAGTTAATACTCGAGTATTAACTGGTCTGGTAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTACCAGACCAGTTAATACTCGAGTATTAACTGGTCTGGTAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000127808