Construct: shRNA TRCN0000330242

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014872.2-2593s21c1
DNA Barcode:
ACCTTCTGATGGGAGTATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZBTB5 (9925)
Gene Description:
zinc finger and BTB domain containing 5
Transcript:
RefSeq NM_014872.2 (NON-CURRENT)
Match location:
Position 2593 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000330242)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTTCTGATGGGAGTATTAT
Hairpin Sequence:
5'-CCGG-ACCTTCTGATGGGAGTATTAT-CTCGAG-ATAATACTCCCATCAGAAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTTCTGATGGGAGTATTATCTCGAGATAATACTCCCATCAGAAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTTCTGATGGGAGTATTATCTCGAGATAATACTCCCATCAGAAGGT-3'

Other clones with same target sequence:

(none)