Construct: shRNA TRCN0000330340

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020187.2-1202s21c1
DNA Barcode:
GATAATAGGTTCTTAACATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HMCES (56941)
Gene Description:
5-hydroxymethylcytosine binding, ES cell specific
Transcript:
RefSeq NM_020187.2 (NON-CURRENT)
Match location:
Position 1202 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000330340)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATAATAGGTTCTTAACATTG
Hairpin Sequence:
5'-CCGG-GATAATAGGTTCTTAACATTG-CTCGAG-CAATGTTAAGAACCTATTATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATAATAGGTTCTTAACATTGCTCGAGCAATGTTAAGAACCTATTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATAATAGGTTCTTAACATTGCTCGAGCAATGTTAAGAACCTATTATC-3'

Other clones with same target sequence:

(none)