Construct: shRNA TRCN0000330360

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017784.3-2247s21c1
DNA Barcode:
GACAGTGATATTCCACACGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OSBPL10 (114884)
Gene Description:
oxysterol binding protein like 10
Transcript:
RefSeq NM_017784.3 (NON-CURRENT)
Match location:
Position 2247 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000330360)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAGTGATATTCCACACGAA
Hairpin Sequence:
5'-CCGG-GACAGTGATATTCCACACGAA-CTCGAG-TTCGTGTGGAATATCACTGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAGTGATATTCCACACGAACTCGAGTTCGTGTGGAATATCACTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAGTGATATTCCACACGAACTCGAGTTCGTGTGGAATATCACTGTC-3'

Other clones with same target sequence:

  1. TRCN0000149806