Construct: shRNA TRCN0000330384

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025180.3-2285s21c1
DNA Barcode:
GACTTCCTACTCTCTATGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CEP63 (80254)
Gene Description:
centrosomal protein 63
Transcript:
RefSeq NM_025180.3 (NON-CURRENT)
Match location:
Position 2285 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000330384)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTTCCTACTCTCTATGTAA
Hairpin Sequence:
5'-CCGG-GACTTCCTACTCTCTATGTAA-CTCGAG-TTACATAGAGAGTAGGAAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTTCCTACTCTCTATGTAACTCGAGTTACATAGAGAGTAGGAAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTTCCTACTCTCTATGTAACTCGAGTTACATAGAGAGTAGGAAGTC-3'

Other clones with same target sequence:

  1. TRCN0000133672