Construct: shRNA TRCN0000330599

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138775.2-2141s21c1
DNA Barcode:
TTACCTGAACACATCATATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ALKBH8 (91801)
Gene Description:
alkB homolog 8, tRNA methyltransferase
Transcript:
RefSeq NM_138775.2 (NON-CURRENT)
Match location:
Position 2141 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000330599)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTACCTGAACACATCATATAT
Hairpin Sequence:
5'-CCGG-TTACCTGAACACATCATATAT-CTCGAG-ATATATGATGTGTTCAGGTAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTACCTGAACACATCATATATCTCGAGATATATGATGTGTTCAGGTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTACCTGAACACATCATATATCTCGAGATATATGATGTGTTCAGGTAA-3'

Other clones with same target sequence:

(none)