Construct: shRNA TRCN0000330631

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018318.3-1142s21c1
DNA Barcode:
GAAGAGCAGAAACGAAGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC91 (55297)
Gene Description:
coiled-coil domain containing 91
Transcript:
RefSeq NM_018318.3 (NON-CURRENT)
Match location:
Position 1142 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000330631)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAGCAGAAACGAAGTGAA
Hairpin Sequence:
5'-CCGG-GAAGAGCAGAAACGAAGTGAA-CTCGAG-TTCACTTCGTTTCTGCTCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAGCAGAAACGAAGTGAACTCGAGTTCACTTCGTTTCTGCTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAGCAGAAACGAAGTGAACTCGAGTTCACTTCGTTTCTGCTCTTC-3'

Other clones with same target sequence:

  1. TRCN0000130878