Construct: shRNA TRCN0000330750

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022662.2-1594s21c1
DNA Barcode:
GCTCCAGTTACGCTGTGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANAPC1 (64682)
Gene Description:
anaphase promoting complex subunit 1
Transcript:
RefSeq NM_022662.2 (NON-CURRENT)
Match location:
Position 1594 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000330750)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCAGTTACGCTGTGTAAA
Hairpin Sequence:
5'-CCGG-GCTCCAGTTACGCTGTGTAAA-CTCGAG-TTTACACAGCGTAACTGGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCAGTTACGCTGTGTAAACTCGAGTTTACACAGCGTAACTGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCAGTTACGCTGTGTAAACTCGAGTTTACACAGCGTAACTGGAGC-3'

Other clones with same target sequence:

  1. TRCN0000163532