Construct: shRNA TRCN0000330909

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138570.2-1059s21c1
DNA Barcode:
CATATTTGCTTCCTCCCTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC38A10 (124565)
Gene Description:
solute carrier family 38 member 10
Transcript:
RefSeq NM_138570.2 (NON-CURRENT)
Match location:
Position 1059 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000330909)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATATTTGCTTCCTCCCTTAA
Hairpin Sequence:
5'-CCGG-CATATTTGCTTCCTCCCTTAA-CTCGAG-TTAAGGGAGGAAGCAAATATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATATTTGCTTCCTCCCTTAACTCGAGTTAAGGGAGGAAGCAAATATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATATTTGCTTCCTCCCTTAACTCGAGTTAAGGGAGGAAGCAAATATG-3'

Other clones with same target sequence:

  1. TRCN0000151835