Construct: shRNA TRCN0000330921

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015110.3-3711s21c1
DNA Barcode:
TACAGTGGAAGAAGGGTTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMC5 (23137)
Gene Description:
structural maintenance of chromosomes 5
Transcript:
RefSeq NM_015110.3 (NON-CURRENT)
Match location:
Position 3711 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000330921)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACAGTGGAAGAAGGGTTAAT
Hairpin Sequence:
5'-CCGG-TACAGTGGAAGAAGGGTTAAT-CTCGAG-ATTAACCCTTCTTCCACTGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACAGTGGAAGAAGGGTTAATCTCGAGATTAACCCTTCTTCCACTGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACAGTGGAAGAAGGGTTAATCTCGAGATTAACCCTTCTTCCACTGTA-3'

Other clones with same target sequence:

(none)