Construct: shRNA TRCN0000330925

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032182.3-1523s21c1
DNA Barcode:
TGGTGCGATTCATGGATATAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABRAXAS2 (23172)
Gene Description:
abraxas 2, BRISC complex subunit
Transcript:
RefSeq NM_032182.3 (NON-CURRENT)
Match location:
Position 1523 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000330925)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTGCGATTCATGGATATAC
Hairpin Sequence:
5'-CCGG-TGGTGCGATTCATGGATATAC-CTCGAG-GTATATCCATGAATCGCACCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTGCGATTCATGGATATACCTCGAGGTATATCCATGAATCGCACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTGCGATTCATGGATATACCTCGAGGTATATCCATGAATCGCACCA-3'

Other clones with same target sequence:

(none)