Construct: shRNA TRCN0000331044

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017847.4-700s21c1
DNA Barcode:
CTGCTACTTCTGTCAGCTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ODR4 (54953)
Gene Description:
odr-4 GPCR localization factor homolog
Transcript:
RefSeq NM_017847.4 (NON-CURRENT)
Match location:
Position 700 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000331044)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCTACTTCTGTCAGCTATA
Hairpin Sequence:
5'-CCGG-CTGCTACTTCTGTCAGCTATA-CTCGAG-TATAGCTGACAGAAGTAGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCTACTTCTGTCAGCTATACTCGAGTATAGCTGACAGAAGTAGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCTACTTCTGTCAGCTATACTCGAGTATAGCTGACAGAAGTAGCAG-3'

Other clones with same target sequence:

  1. TRCN0000136485