Construct: shRNA TRCN0000331115

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016937.2-4140s21c1
DNA Barcode:
GCCTGCATGAAAGCTACACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POLA1 (5422)
Gene Description:
DNA polymerase alpha 1, catalytic subunit
Transcript:
RefSeq NM_016937.2 (NON-CURRENT)
Match location:
Position 4140 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000331115)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGCATGAAAGCTACACTT
Hairpin Sequence:
5'-CCGG-GCCTGCATGAAAGCTACACTT-CTCGAG-AAGTGTAGCTTTCATGCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGCATGAAAGCTACACTTCTCGAGAAGTGTAGCTTTCATGCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGCATGAAAGCTACACTTCTCGAGAAGTGTAGCTTTCATGCAGGC-3'

Other clones with same target sequence:

  1. TRCN0000052957
  2. TRCN0000174228