Construct: shRNA TRCN0000331208
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_170738.2-368s21c1
- DNA Barcode:
- GCCCGCATCAAAGCTCAGGAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MRPL11 (65003)
- Gene Description:
- mitochondrial ribosomal protein L11
- Transcript:
- RefSeq NM_170738.2 (NON-CURRENT)
- Match location:
- Position 368 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000331208)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCCGCATCAAAGCTCAGGAT
- Hairpin Sequence:
-
5'-CCGG-GCCCGCATCAAAGCTCAGGAT-CTCGAG-ATCCTGAGCTTTGATGCGGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCCGCATCAAAGCTCAGGATCTCGAGATCCTGAGCTTTGATGCGGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCCGCATCAAAGCTCAGGATCTCGAGATCCTGAGCTTTGATGCGGGC-3'
Other clones with same target sequence:
-
TRCN0000117466
