Construct: shRNA TRCN0000331253

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015982.3-518s21c1
DNA Barcode:
GCGCAGAAGCCACTAATGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
YBX2 (51087)
Gene Description:
Y-box binding protein 2
Transcript:
RefSeq NM_015982.3 (NON-CURRENT)
Match location:
Position 518 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000331253)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCAGAAGCCACTAATGTAA
Hairpin Sequence:
5'-CCGG-GCGCAGAAGCCACTAATGTAA-CTCGAG-TTACATTAGTGGCTTCTGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCAGAAGCCACTAATGTAACTCGAGTTACATTAGTGGCTTCTGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCAGAAGCCACTAATGTAACTCGAGTTACATTAGTGGCTTCTGCGC-3'

Other clones with same target sequence:

  1. TRCN0000107507