Construct: shRNA TRCN0000331266

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020738.2-5082s21c1
DNA Barcode:
GGCCTGCAAGATCCAATTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIDINS220 (57498)
Gene Description:
kinase D interacting substrate 220
Transcript:
RefSeq NM_020738.2 (NON-CURRENT)
Match location:
Position 5082 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000331266)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCCTGCAAGATCCAATTATA
Hairpin Sequence:
5'-CCGG-GGCCTGCAAGATCCAATTATA-CTCGAG-TATAATTGGATCTTGCAGGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCCTGCAAGATCCAATTATACTCGAGTATAATTGGATCTTGCAGGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCCTGCAAGATCCAATTATACTCGAGTATAATTGGATCTTGCAGGCC-3'

Other clones with same target sequence:

(none)