Construct: shRNA TRCN0000331339

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020948.3-809s21c1
DNA Barcode:
ATCCGCCCACGTCGATGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MIER1 (57708)
Gene Description:
MIER1 transcriptional regulator
Transcript:
RefSeq NM_020948.3 (NON-CURRENT)
Match location:
Position 809 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000331339)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCCGCCCACGTCGATGTAAA
Hairpin Sequence:
5'-CCGG-ATCCGCCCACGTCGATGTAAA-CTCGAG-TTTACATCGACGTGGGCGGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCCGCCCACGTCGATGTAAACTCGAGTTTACATCGACGTGGGCGGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCCGCCCACGTCGATGTAAACTCGAGTTTACATCGACGTGGGCGGAT-3'

Other clones with same target sequence:

(none)