Construct: shRNA TRCN0000331351

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000749.3-1638s21c1
DNA Barcode:
ATGGGAGAAACTCTGGTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHRNB3 (1142)
Gene Description:
cholinergic receptor nicotinic beta 3 subunit
Transcript:
RefSeq NM_000749.3 (NON-CURRENT)
Match location:
Position 1638 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000331351)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGGAGAAACTCTGGTAAAT
Hairpin Sequence:
5'-CCGG-ATGGGAGAAACTCTGGTAAAT-CTCGAG-ATTTACCAGAGTTTCTCCCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGGAGAAACTCTGGTAAATCTCGAGATTTACCAGAGTTTCTCCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGGAGAAACTCTGGTAAATCTCGAGATTTACCAGAGTTTCTCCCAT-3'

Other clones with same target sequence:

(none)