Construct: shRNA TRCN0000331628

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145367.3-1677s21c1
DNA Barcode:
GCGGCACTGTTGACTTAGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Txndc5 (105245)
Gene Description:
thioredoxin domain containing 5
Transcript:
RefSeq NM_145367.3 (NON-CURRENT)
Match location:
Position 1677 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000331628)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGCACTGTTGACTTAGTAA
Hairpin Sequence:
5'-CCGG-GCGGCACTGTTGACTTAGTAA-CTCGAG-TTACTAAGTCAACAGTGCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGCACTGTTGACTTAGTAACTCGAGTTACTAAGTCAACAGTGCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGCACTGTTGACTTAGTAACTCGAGTTACTAAGTCAACAGTGCCGC-3'

Other clones with same target sequence:

  1. TRCN0000099700