Construct: shRNA TRCN0000331713

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009058.2-2021s21c1
DNA Barcode:
GCCTGCAACAACTACAGCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ralgds (19730)
Gene Description:
ral guanine nucleotide dissociation stimulator
Transcript:
RefSeq NM_009058.2 (CURRENT)
Match location:
Position 2021 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000331713)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGCAACAACTACAGCATT
Hairpin Sequence:
5'-CCGG-GCCTGCAACAACTACAGCATT-CTCGAG-AATGCTGTAGTTGTTGCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGCAACAACTACAGCATTCTCGAGAATGCTGTAGTTGTTGCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGCAACAACTACAGCATTCTCGAGAATGCTGTAGTTGTTGCAGGC-3'

Other clones with same target sequence:

  1. TRCN0000055073