Construct: shRNA TRCN0000331916

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021886.1-238s21c3
DNA Barcode:
GCTCTTAGAATATAAGTCCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cenph (26886)
Gene Description:
centromere protein H
Transcript:
RefSeq NM_021886.1 (NON-CURRENT)
Match location:
Position 238 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000331916)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTTAGAATATAAGTCCAT
Hairpin Sequence:
5'-CCGG-GCTCTTAGAATATAAGTCCAT-CTCGAG-ATGGACTTATATTCTAAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTTAGAATATAAGTCCATCTCGAGATGGACTTATATTCTAAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTTAGAATATAAGTCCATCTCGAGATGGACTTATATTCTAAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000072098