Construct: shRNA TRCN0000331960

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004721.1-1226s21c1
DNA Barcode:
CCTGCTCATCTCTGACTACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pigu (228812)
Gene Description:
phosphatidylinositol glycan anchor biosynthesis, class U
Transcript:
RefSeq NM_001004721.1 (CURRENT)
Match location:
Position 1226 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000331960)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCTCATCTCTGACTACTT
Hairpin Sequence:
5'-CCGG-CCTGCTCATCTCTGACTACTT-CTCGAG-AAGTAGTCAGAGATGAGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCTCATCTCTGACTACTTCTCGAGAAGTAGTCAGAGATGAGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCTCATCTCTGACTACTTCTCGAGAAGTAGTCAGAGATGAGCAGG-3'

Other clones with same target sequence:

  1. TRCN0000124973