Construct: shRNA TRCN0000332028

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024183.5-1009s21c1
DNA Barcode:
CGTGCAAATGAGAACAGCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fip1l1 (66899)
Gene Description:
FIP1 like 1 (S. cerevisiae)
Transcript:
RefSeq NM_024183.5 (CURRENT)
Match location:
Position 1009 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000332028)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGCAAATGAGAACAGCAAT
Hairpin Sequence:
5'-CCGG-CGTGCAAATGAGAACAGCAAT-CTCGAG-ATTGCTGTTCTCATTTGCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGCAAATGAGAACAGCAATCTCGAGATTGCTGTTCTCATTTGCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGCAAATGAGAACAGCAATCTCGAGATTGCTGTTCTCATTTGCACG-3'

Other clones with same target sequence:

  1. TRCN0000123576