Construct: shRNA TRCN0000332135

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008851.3-2888s21c1
DNA Barcode:
GCAAACCCATTCGAGTCTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pitpnm1 (18739)
Gene Description:
phosphatidylinositol transfer protein, membrane-associated 1
Transcript:
RefSeq NM_008851.3 (NON-CURRENT)
Match location:
Position 2888 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000332135)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAACCCATTCGAGTCTCTT
Hairpin Sequence:
5'-CCGG-GCAAACCCATTCGAGTCTCTT-CTCGAG-AAGAGACTCGAATGGGTTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAACCCATTCGAGTCTCTTCTCGAGAAGAGACTCGAATGGGTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAACCCATTCGAGTCTCTTCTCGAGAAGAGACTCGAATGGGTTTGC-3'

Other clones with same target sequence:

  1. TRCN0000100732