Construct: shRNA TRCN0000332353

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134080.1-8189s21c1
DNA Barcode:
GCCCAAATCAAGACTCTTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Flnb (286940)
Gene Description:
filamin, beta
Transcript:
RefSeq NM_134080.1 (CURRENT)
Match location:
Position 8189 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000332353)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAAATCAAGACTCTTAAT
Hairpin Sequence:
5'-CCGG-GCCCAAATCAAGACTCTTAAT-CTCGAG-ATTAAGAGTCTTGATTTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAAATCAAGACTCTTAATCTCGAGATTAAGAGTCTTGATTTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAAATCAAGACTCTTAATCTCGAGATTAAGAGTCTTGATTTGGGC-3'

Other clones with same target sequence:

  1. TRCN0000091373