Construct: shRNA TRCN0000332354

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153058.3-292s21c1
DNA Barcode:
GCAGGCATGACATCATTGCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mapre2 (212307)
Gene Description:
microtubule-associated protein, RP/EB family, member 2
Transcript:
RefSeq NM_153058.3 (NON-CURRENT)
Match location:
Position 292 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000332354)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGCATGACATCATTGCAT
Hairpin Sequence:
5'-CCGG-GCAGGCATGACATCATTGCAT-CTCGAG-ATGCAATGATGTCATGCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGCATGACATCATTGCATCTCGAGATGCAATGATGTCATGCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGCATGACATCATTGCATCTCGAGATGCAATGATGTCATGCCTGC-3'

Other clones with same target sequence:

  1. TRCN0000088126