Construct: shRNA TRCN0000332448

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145632.2-210s21c1
DNA Barcode:
CCTCATCTTAGATGTAAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Polr2h (245841)
Gene Description:
polymerase (RNA) II (DNA directed) polypeptide H
Transcript:
RefSeq NM_145632.2 (CURRENT)
Match location:
Position 210 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000332448)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCATCTTAGATGTAAACAT
Hairpin Sequence:
5'-CCGG-CCTCATCTTAGATGTAAACAT-CTCGAG-ATGTTTACATCTAAGATGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCATCTTAGATGTAAACATCTCGAGATGTTTACATCTAAGATGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCATCTTAGATGTAAACATCTCGAGATGTTTACATCTAAGATGAGG-3'

Other clones with same target sequence:

  1. TRCN0000111334