Construct: shRNA TRCN0000332538

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172473.2-2551s21c1
DNA Barcode:
CCGGAAATTGATGTAAATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hace1 (209462)
Gene Description:
HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1
Transcript:
RefSeq NM_172473.2 (NON-CURRENT)
Match location:
Position 2551 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000332538)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGAAATTGATGTAAATGAT
Hairpin Sequence:
5'-CCGG-CCGGAAATTGATGTAAATGAT-CTCGAG-ATCATTTACATCAATTTCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGAAATTGATGTAAATGATCTCGAGATCATTTACATCAATTTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGAAATTGATGTAAATGATCTCGAGATCATTTACATCAATTTCCGG-3'

Other clones with same target sequence:

  1. TRCN0000086759