Construct: shRNA TRCN0000332576

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_054050.2-3307s21c1
DNA Barcode:
CCCAGATTATATTGACGGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rapgef1 (107746)
Gene Description:
Rap guanine nucleotide exchange factor (GEF) 1
Transcript:
RefSeq NM_054050.2 (CURRENT)
Match location:
Position 3307 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000332576)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGATTATATTGACGGGAA
Hairpin Sequence:
5'-CCGG-CCCAGATTATATTGACGGGAA-CTCGAG-TTCCCGTCAATATAATCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGATTATATTGACGGGAACTCGAGTTCCCGTCAATATAATCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGATTATATTGACGGGAACTCGAGTTCCCGTCAATATAATCTGGG-3'

Other clones with same target sequence:

  1. TRCN0000098092