Construct: shRNA TRCN0000332638
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_172301.3-827s21c1
- DNA Barcode:
- CCATGTTTATTGCAAGCAAAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Ccnb1 (268697)
- Gene Description:
- cyclin B1
- Transcript:
- RefSeq NM_172301.3 (CURRENT)
- Match location:
- Position 827 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000332638)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCATGTTTATTGCAAGCAAAT
- Hairpin Sequence:
-
5'-CCGG-CCATGTTTATTGCAAGCAAAT-CTCGAG-ATTTGCTTGCAATAAACATGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCATGTTTATTGCAAGCAAATCTCGAGATTTGCTTGCAATAAACATGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCATGTTTATTGCAAGCAAATCTCGAGATTTGCTTGCAATAAACATGG-3'
Other clones with same target sequence:
-
TRCN0000087925
